Synthesis Docking Studies, Characterization and Anti-tubercular Activity of Ofloxacin Containing Thiazolidinone Derivatives

Background: Ofloxacin is a fluoroquinolone antibiotic very useful in the treatment of several bacterial infections. The Thiazolidinone ring system represents privileged structure drug discovery. multi-drug-resistant tuberculosis bacteria infects many people worldwide and disease kills 1.8 million annually, so combination ofloxacin-thiazolidinone gives great promise for future potential antitubercular drugs. Methods: Ofloxacin treated with hydrazine hydrate results form ofloxacin hydrazides. These hydrazides were various aromatic aldehydes containing Schiff bases. compounds are thioglycolic acid presence zinc chloride, DMF as solvent forms thiazolidinone derivatives. derivatives (T1, T2, T3, T4, T5, T6) purified using Ethanol by recrystallization procedure. Chimera software used protein stabilization. 5BS8 was downloaded from PDB. Results: these characterized TLC, melting point, solubility Spectroscopic methods like FT-IR, 1H-NMR HRMS. Predicted in silico studies Autodock vina, which academic free version performed action synthesized standard concentrations prepared (0.8, 1.6, 3.12, 6.25, 12.5, 25, 50, 100 μg/ml) Assay Microplate Alamar Blue method (MABA method). Docking done autodock vina software. Conclusion: MIC values reveal that all show activity concentration 1.6 μg/ml compared to docking T1-T6 showed good binding energies above standard, potent agents. justify;">Key words: Ofloxacin, Thiazolidinone, Antitubercular activity, studies, MABA method, Vina.